A pilot grey water treatment system and collection network were designed, installed, and operated in Jordan Valley using natural filtration materials. Grey water from showers and washing sinks was collected from four houses. In order to evaluate the performance of multi-layer filter (MLF) ability to remove the pollutants from the collected grey water, the quality of treated and untreated grey water was examined and the suitability of treated grey water for irrigation was assessed. The results revealed that the efficiency removal of organic material before UV disinfection stage for BOD5, COD, and TSS was about 88.6, 83, and 92.2%, respectively. The efficiency removal rates for nutrients by MLF were a bit low as 32.5% of total phosphorous and 19.8% of total nitrogen were removed, whereas the removal efficiency of heavy metals Zn, Fe, and Ba were 94.8, 81.2, and 15.7%, respectively. The results showed that the pilot plant has efficiently reduced the coliform organisms, thermo-tolerance coli count, and Escherichia coli by more than 99.9% removal efficiency by the double-filter stage and 100% after disinfection stage. The current MLF system has the transferability potential to other locations of the developing world.